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Transfection

Electroporation does not enhance chemical or viral gene transfer in vitro.

Published in Gene Therapy Weekly, March 27th, 2003

Electroporation does not enhance chemical or viral gene transfer in vitro.

"To develop improved methods of gene delivery, packaging DNA in chemical or viral vectors could increase electroporation-mediated transfection. To test this hypothesis, electroporation was applied to DU145 prostate cancer cells incubated with green fluorescent protein-encoded DNA plasmid either naked or packaged with cationic lipid (Lipofectin), polycationic peptide (salmon prolamine) or retroviral vectors (Moloney murine leukemia viruses) and then assayed for gene expression and cell viability," wrote A.L. Coulberson and colleagues, Georgia Institute of Technology, School of Chemical...

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