Gene Sequencing
A chromatography method reveals the methylation fingerprints of genes
Published in Gene Therapy Weekly, April 3rd, 2003
According to recent research from France, "the bisulfite genomic sequencing method is one of the most widely used techniques for methylation analysis in heterogeneous unbiased PCR, amplifying for both methylated and unmethylated alleles simultaneously. However it requires labor-intensive and time-consuming cloning and sequencing steps."
"In the current study, we used a denaturing high-performance liquid chromatography (DHPLC) procedure in a complementary way with the bisulfite genomic sequencing to analyze the methylation of differentially methylated regions (DMRs) of imprinted...
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Source: Gene Therapy Weekly (2003-04-03)
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