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Gene Therapy Weekly

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Microbiology



 Leishmania doesn't respond to RNA interference like other microbes do



July 24th, 2003

Leishmania doesn't respond to RNA interference like other microbes do.

According to recent research from the United States, "Approaches which eliminate mRNA expression directly are ideally suited for reverse genetics applications in eukaryotic microbes which are asexual diploids, such as the protozoan parasite Leishmania. RNA interference (RNAi) approaches have been successful in many species, including the related parasite Trypanosoma brucei. For RNAi tests in Leishmania, we developed improved protocols for transient and stable DNA transfection, attaining efficiencies of up to 25 and 3%, respectively."

"This facilitated...


Source: Gene Therapy Weekly (2003-07-24)

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