Vector Development
Bacterial artificial clones are useful for a number of genetic applications
Published in Gene Therapy Weekly, August 14th, 2003
According to published research from England, "bacterial artificial chromosomes (BACs) have many advantages over other large-insert cloning vectors and have been used for a variety of genetic applications, including the final contigs of the human genome. We describe the utilization of a BAC construct to study gene regulation in a tissue culture-based system, using a 170-kb clone containing the entire Wilson disease (WND) locus as a model. A second BAC construct that lacked a putative negatively regulating promoter sequence was created."
"A nonviral method of gene delivery...
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Source: Gene Therapy Weekly (2003-08-14)
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