Published in Gene Therapy Weekly, September 11th, 2003
According to published research from Denmark, "a new cultivation-independent method for studying conjugal gene transfer between bacteria was evaluated. The method was based on direct detection and enumeration of donor and transconjugant bacterial cells by flow cytometry. Specific detection of transconjugants was obtained by using a conjugative plasmid tagged with a reporter gene (gfp) encoding green fluorescent protein."
"A chromosomal encoded repressor (lacI(q1)) repressed expression of GFP in the donor bacteria. Enumeration of the donor cells was performed after induction of GFP expression...
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