Biotechnology
A new technique offers oligonucleotide immobilization for hybridization
Published in Gene Therapy Weekly, October 30th, 2003
According to new research from Turkey, "the aim of this study was to develop a nucleic acid sensor based on piezoelectric crystal microbalance system (QCM) for following hybridization. Piezoelectric quartz crystal surfaces were first treated in a glow-discharge apparatus with ethylene diamine (EDA) plasma at 15 W (discharge power), 2.5 min (incubation time) and 35 ml/min (monomer flow rate) to create amino groups on the crystal surfaces."
"The thickness of the EDA-plasma film formed was about 43±24 Angstrom. Then, the amino groups on the crystal surfaces were converted to...
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Source: Gene Therapy Weekly (2003-10-30)
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