Nucleofection enhances gene transfer into T cells

According to recent research published in the Journal of Immunological Methods, "it has been difficult to assess the role of specific genes in activation and differentiation of peripheral T cell subsets such as naive, effector and memory T cells due to the impairments in T cell development and immune pathologies often observed in genetically manipulated mouse models, and the lack of reliable methods for introducing genes into primary mouse T cells."

"In this study, we demonstrate transient transfection of genes into resting and activated mouse CD4 T cell subsets using ''nucleofection'', a...

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