Polycation-mediated precipitation captures plasmid DNA from alkaline lysate

"The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Large-scale purification of plasmid DNA from bacterial cell culture normally includes one or several chromatographic steps. Prechromatographic steps include precipitation with solvents, salts, and polymers combined with enzymatic degradation of nucleic acids," scientists in Sweden report.

"No method alone has so far been able to selectively capture plasmid DNA directly from a clarified alkaline lysate," according to P.O. Wahlund...

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