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Stem Cell Research

Competitive repopulation assay monitors stem cell progeny after transplantation

Published in Gene Therapy Weekly, March 3rd, 2005

Competitive repopulation assay monitors stem cell progeny after transplantation

"In multiunit cord blood transplantation, hematopoietic stem cells from each unrelated cord blood (UCB) unit competitively reconstitute the hematopoietic system in a recipient. To evaluate the fate of the progeny of each UCB unit and to determine the effects of graft-versus-graft reaction, we established a novel competitive repopulation assay using non-obese diabetic severe combined immunodeficient gamma chain (NOD/SCID/gammac(null)) mice in which human T lymphocytes develop from CD34+ cells," scientists writing in the journal Molecular Therapy report.

"CD34+ cells...

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