Cloning
pTOC-T vector constructed by using GST-ParE toxin for direct cloning
Published in Gene Therapy Weekly, March 10th, 2005
According to published research from South Korea, "Using linker insertion mutations, we determined the most stable region of the parE gene which encodes a toxic protein (ParE) that inhibits growth of Escherichia coli."
"The toxicity of ParE was sustained until a 144 bp linker was inserted into this region," the scientists announced. "We have developed a 3' T-overhang vector based on these characteristics of the GST-ParE toxin, and named pTOC-T."
"Because pTOC-T uses a post-segregational killing system, all transformants grown up on the plates can...
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Source: Gene Therapy Weekly (2005-03-10)
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