Genetics
Novel protocol improves microarray gene marker identification
Published in Gene Therapy Weekly, August 25th, 2005
"Despite the success and popularity of microarrays as a high-throughput technology for gene expression studies, its sensitivity is as yet fairly limited," noted researchers in Israel, who "successfully combined the use of PCR-Select cDNA subtraction and Affymetrix GeneChips (AGC) to identify differentially expressed gene markers."
"Total RNA (totRNA) from combined hippocampus and cerebellum tissues of 2-week-old rat pups maintained for 5 weeks on an n-3 fatty acid (FA)-deficient diet supplied to dams was isolated, SMART-amplified, and used for PCR-Select subtraction versus...
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Source: Gene Therapy Weekly (2005-08-25)
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