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Genetics & Genomics

A thermoresponsive copolymer vector is suitable for gene transfection

Published in Gene Therapy Weekly, December 8th, 2005

A study from the People's Republic of China has described a thermoresponsive chitosan-NIPAAm/vinyl laurate (VL) copolymer (PNVLCS) vector for gene transfection.

"A carboxyl-terminated N-isopropylacrylamide/VL copolymer was prepared and coupled with chitosan (molecular weight=2000) to produce a PNVLCS vector. The aqueous solution of PNVLCS displayed an obvious thermoresponsive behavior with a lower critical solution temperature (LCST) about 26 degrees C," wrote S.J. Sun and colleagues, Tianjin University.

"Transmission electron microscopy (TEM) showed that the size of PNVLCS/DNA complexes varied with charge ratios (+/-), and the smaller nanoparticles...

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