DNA Research
Interleukin-2 gene complex transfection characterized
Published in Gene Therapy Weekly, August 3rd, 2006
"IL-2 used in the treatment of malignant tumors has an anti-tumor efficacy. In this study, we have studied in vitro characterization and transfection efficiency of a plasmid encoding hIL-2, pCXWN-hIL-2, complexed to chitosan, polyethylenimine or DOTAP with varying ratios," wrote G. Ozgel and colleagues, University of Marmara.
"Plasmid DNA was amplified in Escherichia coli DH5 alpha and isolated by alkali lysis method. The pDNA/chitosan, pDNA/PEI or pDNA/DOTAP complexes were analyzed by agarose gel electrophoresis for...
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Source: Gene Therapy Weekly (2006-08-03)
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