Genetics
Findings from the United States and Japan advance knowledge in genetics research
Published in Gene Therapy Weekly, August 10th, 2006
Study 1: A study from the United States has reported that inclusion of a matrix-attached region in a 7SK pol III vector increases the efficiency of shRNA-mediated gene silencing in embryonic carcinoma cells.
"RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters," wrote V.N. Sotirova and colleagues, University of Connecticut.
"Here we...
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Source: Gene Therapy Weekly (2006-08-10)
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