Published in Gene Therapy Weekly, November 9th, 2006
"We have developed an in situ hybridization procedure for the detection of miRNAs in tissue sections from mouse embryos and adult organs. The method uses highly specific washing conditions for RNA oligonucleotide probes conjugated to a fluorescein hapten," wrote M. Deo and colleagues, University of Michigan.
"We show that this method detects predominantly mature miRNAs rather than the miRNA precursors or primary transcripts. We have determined expression patterns for several miRNAs expressed in...
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