Gene Therapy

New gene therapy data have been reported by researchers at Central Hospital

Published in Gene Therapy Weekly, June 28th, 2007

Fresh data on gene therapy are presented in the report "Cloning and expression of special F protein from human liver." Researchers in Tianjin, People's Republic of China conducted a study "To clone human liver special F protein and to express it in a prokaryotic system. Total RNA was isolated from human liver tissue and first-strand cDNA was reverse transcribed using the PCR reverse primer."

"Following this, cDNA of the F protein was ligated into the clone vector pUCm-T. The segment of F protein's cDNA was subcloned into the expression vector pET-15b and transformed into E. coli BL21 (DE3) pLyss. Isopropy-beta-D-thiogalactoside (IPTG) was then used to induce...

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Source: Gene Therapy Weekly (2007-06-28)

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