Published in Gene Therapy Weekly, November 29th, 2007
"To improve transformation efficiencies, the effect of several factors were examined by measuring transient expression levels of beta-glucuronidase and the number of resistant explants with PPT selection. The hypervirulent Agrobacterium tumefaciens strain KYRT1 proved to be a better transformer than EHA105 and LBA4404," wrote W. Dang and colleagues.
The researchers concluded: "Improved transformation efficiencies were obtained when embryonic tips were incubated...
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Source: Gene Therapy Weekly (2007-11-29)
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