Gene Therapy
Research on gene therapy published by scientists at Max Planck Institute for Infection Biology
Published in Gene Therapy Weekly, March 27th, 2008
Scientists discuss in 'Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning' new findings in gene. According to recent research from Berlin, Germany, "We have developed an efficient strategy that combines immunoglobulin (Ig) gene repertoire analysis and Ig reactivity profiling at the single cell level. Based on surface marker expression individual cells at different stages of human B cell development are isolated by fluorescence-activated cell sorting."
"For each cell Ig heavy and corresponding Ig light chain gene transcripts are amplified by nested RT-PCR and cloned into eukaryotic expression...
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Source: Gene Therapy Weekly (2008-03-27)
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