DNA Research

New findings reported from A. Sinha and co-authors describe advances in DNA research

Published in Gene Therapy Weekly, May 8th, 2008

According to recent research published in the Journal of Bacteriology, "Mycobacterium tuberculosis PhoP regulates the expression of unknown virulence determinants and the biosynthesis of complex lipids. PhoP, like other members of the OmpR family, comprises a phosphorylation domain at the amino-terminal half and a DNA-binding domain at the carboxy-terminal half of the protein."

"To explore structural effect of protein phosphorylation and to examine effect of phosphorylation on DNA binding, purified PhoP was phosphorylated by acetyl phosphate in a reaction that was dependent on Mg2+ and Asp-71. Protein phosphorylation was not required for DNA binding; however,...

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Source: Gene Therapy Weekly (2008-05-08)

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