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DNA Research
Investigators at University of Hong Kong zero in on DNA research
May 15th, 2008
"Southwestern blotting is used to investigate DNA-protein interactions. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor," scientists writing in the journal Nature Protocols report. "This method combines the features of Southern and Western blotting techniques; a denaturing SDS-PAGE is first employed to separate proteins electrophoretically based on size, and after transferring the proteins to a membrane support, the membrane-bound proteins are renatured and incubated with a P-32-labeled...
Source: Gene Therapy Weekly (2008-05-15)
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