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Gene Therapy
Research findings from S. Broussau et al update understanding of gene therapy
May 29th, 2008
"We have developed new packaging cell lines (293SFPacLV) that can produce lentiviral vectors (LVs) in serum-free suspension cultures. A cell line derived from 293SF cells, expressing the repressor (CymR) of the cumate switch and the reverse transactivator (rtTA2S-M2) of the tetracycline (Tet) switch, was established first," scientists in Montreal, Canada report. "We next generated clones stably expressing the Gag/Pol and Rev genes of human immunodeficiency virus-1, and the glycoprotein of vesicular stomatitis virus (VSV-G). Expression of Rev and VSV-G was tightly regulated by the cumate and Tet switches. Our best packaging cells produced up to 2.6 x 10(7) transducing...
Source: Gene Therapy Weekly (2008-05-29)
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