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Gene Therapy
Report summarizes gene therapy study findings from National University
July 17th, 2008
"Methylation status of CpG dinucleotides in the promoter/regulatory region contributes to regulation of transcriptional activities of downstream genes. Nearly all plasmid vectors used in mammalian cells are generated from transformed Escherichia coli. However, these E. coli hosts may have different DNA methylation activities," researchers in Taipei, Taiwan report. "For instance E coli JM109 and DH5 alpha contain Dam and Dcm methylases, which are absent in E coli JM110 and GM2163. It has not been determined whether plasmids propagated from E coli of different methylation activities result in altered expression in mammalian cells when transient transfection is conducted....
Source: Gene Therapy Weekly (2008-07-17)
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