Prostate Cancer

Researchers at University of Cambridge target prostate cancer

Published in Gene Therapy Weekly, September 11th, 2008

According to recent research from the United Kingdom, "To investigate the feasibility of transcript profiling in diagnostic formalin-fixed and paraffin-embedded (FFPE) biopsies for prostate cancer. Laser-capture microdissection (LCM) was used to microdissect glandular epithelium as well as stromal tissue in archival prostate needle biopsies."

"Optimized RNA extraction, reverse transcription and real-time PCR (QPCR) protocols were used to detect transcript expression. RNA degradation effects were assessed using hydrolysed cell line RNA and matched xenograft FFPE and frozen tumours. LCM and RNA extraction was achieved in all biopsies from a pilot cohort of five patients....

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Source: Gene Therapy Weekly (2008-09-11)

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