Antisense Technology

Research from G.S. Xu and colleagues has provided new data on antisense technology

Published in Gene Therapy Weekly, November 6th, 2008

According to a study from Huzhou, People's Republic of China, " To elucidate if Fas/FasL signal pathway participates in the immune escape of tumor cells, and if contemporarily preventing Fas/FasL and TNF-induced apoptosis is better for immune cells survival than just blocking Fas/FasL-induced apoptotic signal. Suppression of FasL expression in mouse H22 hepatocellular cancer cells by siRNA technique."

"Wild-type Ad5 14.7K gene was amplified by PCR and transduced into Jurkat T cells. Detecting apoptosis of target Jurkat cells by Flow Cytometry. Detection of TNF-alpha in the culture supernatant of H22 cells by ELISA. FasL and 14.7K gene expression in stably transfected...

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Source: Gene Therapy Weekly (2008-11-06)

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