Gene Therapy

New gene therapy research from A. Nakanishi et al outlined

Published in Gene Therapy Weekly, November 27th, 2008

According to a study from Japan, "Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian Virus 40 (SV40) T antigen; DNA for the expression of SV40 capsid proteins, and vector DNA harboring a reporter gene expression cassette carrying a SV40 origin. The vector DNA harbors a minimal sequence Originating from SV40, and thus can carry a longer transgene."

"Moreover the viable recombinants are not detectable in the vector preparation, and the vectors can transduce the DNA with efficiency similar to. that of virions. Vector particles bearing capsid proteins of BK virus, JC virus, and B-lymphotropic papovavirus...

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Source: Gene Therapy Weekly (2008-11-27)

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