Published in Gene Therapy Weekly, January 8th, 2009
"Using the elongation factor 1-alpha promoter as a test ubiquitous promoter, we evaluated the sequence of the vector, polyadenylation signals, cleavage signals, and ribosome entry site sequences. In ascidian embryos, the most important sequence element for efficient expression was the polyadenylation signal. The polyadenylation signal sequence derived from SV40 and...
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