Gene Therapy

Investigators at Konan University zero in on gene therapy

Published in Gene Therapy Weekly, January 8th, 2009

According to a study from Kobe, Japan, "An expression system for transgenes is an important tool for gene function analyses. The present study describes a promoter sequence that can drive ubiquitous expression in ascidian development and optimization of a plasmid vector for maximizing transgene expression."

"Using the elongation factor 1-alpha promoter as a test ubiquitous promoter, we evaluated the sequence of the vector, polyadenylation signals, cleavage signals, and ribosome entry site sequences. In ascidian embryos, the most important sequence element for efficient expression was the polyadenylation signal. The polyadenylation signal sequence derived from SV40 and...

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Source: Gene Therapy Weekly (2009-01-08)

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