Gene Therapy

Research from X.D. Zhang and co-authors yields new data on gene therapy

Published in Gene Therapy Weekly, February 19th, 2009

"Presently most techniques for gene expression analysis are based on a dye label. Here we describe a novel method for comparing gene expression levels among various tissues or cells by sequence-tagged reverse-transcription PCR coupled with pyrosequencing (termed ''SRPP'')," researchers in Nanjing, People's Republic of China report.

"This method includes three steps: (i) reverse transcription of mRNA with sources-specific RT primers consisting of a tail at the 5'-end for supplying a common PCR priming site, a source-specific sequence in the middle, and a poly-T stretch plus several degenerate bases at the X-end for annealing the mRNA strand. (ii) PCR amplification of the...

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Source: Gene Therapy Weekly (2009-02-19)

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