Published in Gene Therapy Weekly, February 19th, 2009
"This method includes three steps: (i) reverse transcription of mRNA with sources-specific RT primers consisting of a tail at the 5'-end for supplying a common PCR priming site, a source-specific sequence in the middle, and a poly-T stretch plus several degenerate bases at the X-end for annealing the mRNA strand. (ii) PCR amplification of the...
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