Gene Therapy
Studies from Purdue University reveal new findings on gene therapy
Published in Gene Therapy Weekly, February 19th, 2009
"We examined 1,25 dihydroxyvitamin D (1,25(OH)(2)D-3)-induced expression of 25-hydroxyvitamin D-3 24-hydroxylase (CYP24) and apical calcium channel (TRPV6) mRNA levels in 2-, 9-, and 15-day cultures Caco-2 cells that model proliferating, post-proliferative, and differentiated enterocytes. 1,25(OH)(2)D-3-induced (10 nM, 8 h) CYP24 and TRPV6 mRNA levels were significantly greater in differentiated and post-proliferative than proliferating Caco-2 cells (> 16X and > 3X, respectively). Neither CYP24 mRNA half-life nor induction of a - 298 bp rat CYP24 promoter-luciferase reporter construct (10 nM 1,25(OH)(2)D-3, 24 h) were different between proliferating and post-proliferating Caco-2...
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Source: Gene Therapy Weekly (2009-02-19)
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