Researchers from University of California detail new studies and findings in the area of life sciences

New research, 'Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples,' is the subject of a report. "Ethidium monoazide bromide (EMA) treatment of pure culture and environmental waters at low concentrations (1.0-7.5 microg/ml) indicated effective enumeration of viable and viable but nonculturable Escherichia coli in pure cultures, creek waters, and secondary activated sludge effluent samples by quantitative polymerase chain reaction (qPCR) amplification of the uidA and fliC gene targets at turbidity values <10 NTU. However, EMA treatment was not effective in primary clarifier and secondary trickling filter...

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