Gene Therapy

Research from University of Oxford in gene therapy provides new insights

Published in Gene Therapy Weekly, March 12th, 2009

"We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the human papillomavirus E6 or enhanced green fluorescent protein genes," investigators in Oxford, the United Kingdom report.

"Having confirmed siRNA could not act as primer for amplicon generation, the lowest competing primer concentration yielding a linear relationship between template DNA amount (0.1-50 ng) and cycle of threshold (Ct) was determined (6.25 nM). Under these...

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Source: Gene Therapy Weekly (2009-03-12)

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