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Gene Therapy

Scientists at University of Pittsburgh, Medical Department detail research in gene therapy

Published in Gene Therapy Weekly, March 12th, 2009

"Herpes simplex virus type 1 (HSV-1) represents an attractive vehicle for a variety of gene therapy applications. To render this virus safe for clinical use, its cytotoxic genes must be removed without losing its ability to express transgenes efficiently," scientists writing in the journal Biotechnology and Bioengineering report.

"Our vectors are deleted for the essential immediate early genes ICP4 and ICP27. These genes are controlled by unique promoters having enhancer elements responsive to a viral structural protein VP16. The expression of these genes occurs prior to the activation of all other lytic functions and is thus required to initiate and complete the...

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