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Bioengineering
Study results from K. Kimura et al provide new insights into bioengineering
July 9th, 2009
"An industrial strain of Bacillus subtilis (natto) was used to produce poly-gamma-DL-glutamate (gamma PGA), a polymer Of DL-glutamate linked by a gamma-peptide bond. In spite of efforts to improve gamma PGA production by modifying the medium, little attention has been paid to the expression of the gamma PGA synthetase gene," researchers in Tsukuba, Japan report. "In this study, we investigated the expression of the gamma PGA synthetic gene and the gamma PGA product under various conditions with the LacZ-fusion of the synthetic gene (pgsB-lacZ). The 5' upstream regulatory region of the pgsB gene was also investigated by constructing deletion mutations of lacZ-fusion. The...
Source: Gene Therapy Weekly (2009-07-09)
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