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New life sciences research reported from Konkuk University

Published in Gene Therapy Weekly, August 6th, 2009

"Codon optimization was used to synthesize the blh gene from the uncultured marine bacterium 66A03 for expression in Escherichia coli. The expressed enzyme cleaved beta-carotene at its central double bond (15,15') to yield two molecules of all-trans-retinal," scientists in Seoul, South Korea report.

"The molecular mass of the native purified enzyme was similar to 64 kDa as a dimer of 32-kDa subunits. The K-m, k(cat), and k(cat)/K-m values for beta-carotene as substrate were 37 mc M, 3.6 min(-1), and 97 mM(-1) min(-1), respectively. The enzyme exhibited the highest activity for beta-carotene, followed by beta-cryptoxanthin, beta-apo-4'-carotenal, alpha-carotene, and...

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