Published in Gene Therapy Weekly, January 21st, 2010
"Expression of dihydrofolate reductase (DHFR) was markedly increased by folic acid (FA, 50 mu mol/L, 24 h) treatment in endothelial cells. Tetrahydrofolate (THF) is formed after incubation of purified DHFR or cellular extracts with 50 mu mol/L of substrate dihydrofolic acid. THF could then be detected and quantified by high performance liquid chromatography (HPLC) with...
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