Stem Cell Research
Study data from University of California provide new insights into stem cell research
Published in Gene Therapy Weekly, February 11th, 2010
According to recent research from the United States, "Sleeping Beauty (SB) transposon-mediated integration has been shown to achieve long-term transgene expression in a wide range of host cells. In this study, we improved the SB transposon-mediated gene transfer system for transduction of human CD34(+) stem/progenitor cells by two approaches: (1) to increase the transposition efficacy, a hyperactive mutant of SB, HSB, was used; (2) to improve the expression of the SB transposase and the transgene cassette carried by the transposon, different viral and cellular promoters were evaluated."
"SB components were delivered in trans into the target cells by Nucleoporation. The...
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Source: Gene Therapy Weekly (2010-02-11)
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