Gene Therapy
Study results from V. Rakkithawatthana and colleagues in the area of gene therapy published
Published in Gene Therapy Weekly, April 8th, 2010
According to recent research from Pathum Thani, Thailand, "In this study, gene transfection efficiencies using nano-polyplex as gene carriers were investigated in a variety of human cell lines. Gene carriers were prepared using cationic polymer methylated N-(4-pyridinylmethyl) chitosan chloride (MPyMeChC) and/or poly(ethylenimine) (PEI) to form polyplex via electrostatic interaction with 1 mu g of plasmid pGL-3-basic containing CMV promoter/enhancer at different weight ratios."
"Polyplex formations were confirmed by gel retardation assay. The result suggested that packaging of DNA in PEI-containing polyplexes occurred without interference of DNA integrity. Both size and...
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Source: Gene Therapy Weekly (2010-04-08)
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