Life Sciences
Scientists at University of Montreal publish research in life sciences
Published in Gene Therapy Weekly, April 8th, 2010
"The composition of samples obtained upon complexation of DNA with chitosan was analyzed by asymmetrical flow field flow fractionation (AF4) with online UV-visible, multiangle light scattering (MALS), and dynamic light scattering (DLS) detectors. A chitosan labeled with rhodamine B to facilitate UV-vis detection of the polycation was complexed with DNA under conditions commonly used for transfection (chitosan glucosamine to DNA phosphate molar ratio of 5)," researchers in Montreal, Canada report.
"AF4 analysis revealed that 73% of the chitosan-rhodamine remained free ill the dispersion and that the DNA/chitosan complexes had a broad size distribution ranging from 20 to...
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Source: Gene Therapy Weekly (2010-04-08)
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