Recombinant Proteins

Reports outline recombinant proteins study findings from T.R. Lwa and colleagues

Published in Gene Therapy Weekly, June 17th, 2010

According to a study from Singapore, Singapore, "Development of high-throughput functional genomic screening, including si RNA screening, provides a novel approach for quick identification of critical factors involved in biological processes. Here, we apply this strategy to search for cellular genes involved in recombinant protein production."

"Since most of biopharmaceutical proteins are secreted proteins, we develop a cell-based reporter assay using a secreted luciferase. Gaussia luciferase (Gluc), as the reporter. Human embryonic kidney 293 (HEK293) cells transiently transfected with the Gluc reporter plasmid are used to screen our siRNA panel. Three cellular genes,...

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Source: Gene Therapy Weekly (2010-06-17)

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