Published in Gene Therapy Weekly, March 10th, 2011
"This construct (pBAD-Zcal1) was used to transform E. coil cells, and the antisense transcription was induced simply by adding arabinose to the culture medium. We demonstrated that the Zcal1 asttNA effectively silenced lacZ using B-galactosidase activity determinations, SDS-PAGE, and Western blotting. Because the concentration of the lac mRNA was...
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