Antisense Technology

Scientists at University of Bologna Discuss Research in Antisense Technology

Published in Gene Therapy Weekly, March 10th, 2011

2011 MAR 10 -- "Silencing of the lacZ gene in Escherichia coil was attempted by means of the expression of antisense RNAs (asRNAs) in vivo. A short fragment of lacZ was cloned into the pBAD expression vector, in reverse orientation, using the EcoRI and Pstl restriction sites," researchers in Bologna, Italy report.

"This construct (pBAD-Zcal1) was used to transform E. coil cells, and the antisense transcription was induced simply by adding arabinose to the culture medium. We demonstrated that the Zcal1 asttNA effectively silenced lacZ using B-galactosidase activity determinations, SDS-PAGE, and Western blotting. Because the concentration of the lac mRNA was...

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Source: Gene Therapy Weekly (2011-03-10)

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