Hepatocellular Cancer

Recent Studies by S.B. Choi and Co-Authors Add New Data to Hepatocellular Cancer Findings

Published in Gene Therapy Weekly, September 8th, 2011

2011 SEP 8 -- "Hypoxia-inducible factor-1 (HIF-1) is a master transcription factor that plays a central role in the hypoxic expression of various genes. Vascular endothelial growth factor (VEGF), a known target gene of HIF-1 alpha, has been shown to be induced by hypoxia through a HIF-1 alpha-independent pathway," researchers in Gyeonggi Do, South Korea report.

"HIF-1 alpha dominant-negative lentiviral vectors were introduced to decrease the expression of HIF in Hep3B cells. Cells were incubated under normoxic or hypoxic conditions. We performed a VEGF enzyme-linked immunosorbent assay (ELISA) using cell culture supernatants, and Western blotting using cell...

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Source: Gene Therapy Weekly (2011-09-08)

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