Small Interference RNAs (siRNAs)

New Small Interference RNAs (siRNAs) Data Have Been Reported by Investigators at National Taiwan University

Published in Gene Therapy Weekly, February 2nd, 2012

2012 FEB 2 -- According to the authors of recent research from Taipei, Taiwan, "MiR-1 (microRNA-1) has been used as a positive control in some microRNA experiments. We found that miR-1 transfection of nasopharyngeal carcinoma cells reveals a typical apoptotic process as shown by time-lapse microscopy so we investigated the mechanisms of miR-1 inducing apoptosis."

"To confirm that miR-1 induces apoptosis, we used Annexin V and TUNEL staining and caspase assay. To determine that miR-1 directly targets genes that involve in apoptosis, we analyzed microRNA and pathway databases, and cDNA expression microarrays from miR-1 transfected cells. To demonstrate...

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Source: Gene Therapy Weekly (2012-02-02)

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