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Biotechnology

Research on Biotechnology Published by Investigators at Autonomous University

Published in Gene Therapy Weekly, February 23rd, 2012

2012 FEB 23 -- "The culture of HEK-293S cells in a stirred tank bioreactor for adenoviral vectors production for gene therapy is studied. Process monitoring using oxygen uptake rate (OUR) was performed," scientists in Barcelona, Spain report.

"The OUR was determined on-line by the dynamic method, providing good information of the process evolution. OUR enabled cell activity monitoring, facilitating as well the determination of the feeding rate in perfusion cultures and when to infect the culture. Batch cultures were used to validate the monitoring methodology. A cell density of 10 x 10(5) cell/mL was infected, producing 1.3 x 10(9) infectious viral...

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