Life Sciences

Research on life sciences published by scientists at University of Athens

Published in Genetics and Environmental Business Week, March 11th, 2010

According to a study from Athens, Greece, "5382insC frameshift mutation along with 5331G > A (G1738R) missense mutation, both found in exon 20 of the BRCA1 gene, are relatively frequent among the Greek breast and ovarian cancer population (46%). Our goal was to develop a novel, reliable and rapid genotyping/scanning method for mutation detection of the exon 20 of the BRCA1 gene, using high-resolution melting curve analysis."

"The developed methodology was based on real-time PCR and high-resolution melting curve analysis in the presence of LCGreen I dye. Two amplicons on the exon 20 of BRCA1 gene were designed (157 bp and 100 bp), one flanking the exon's boundaries,...

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Source: Genetics and Environmental Business Week (2010-03-11)

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