Protein Therapies
Research on protein therapies reported by scientists at Mahidol University
Published in Genetics and Environmental Health Week, February 17th, 2010
"One of the most crucial steps in mitochondrial isolation is disruption of intact cells to denude intracellular organelles, but the yield and purity of different disruption protocols have not been well addressed. In the present study, MDCK cells were disrupted by mechanical ( sonication and homogenization), physical ( repeated freeze/thaw cycles and hypoosmotic burst), and chemical ( using Triton X-100, NP-40, or CHAPS) methods," scientists in Bangkok, Thailand report.
"Efficacy of cell disruption was evaluated by trypan blue staining and mitochondria were subsequently isolated by standardized differential centrifugation. The yield of isolation was also determined by...
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