Boston University
Engineered riboregulators may be able to control gene expression
Published in Genetics and Environmental Law Weekly, September 18th, 2004
According to recent research published in the journal Nature Biotechnology, "recent studies have demonstrated the important enzymatic, structural and regulatory roles of RNA in the cell. Here we present a post-transcriptional regulation system in Escherichia coli that uses RNA to both silence and activate gene expression. We inserted a complementary cis sequence directly upstream of the ribosome binding site in a target gene."
"Upon transcription, this cis-repressive sequence causes a stem-loop structure to form at the 5'-untranslated region of the mRNA. The stem-loop...
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