Genomics & Genetics Weekly
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Enzymology
Cloned hepatitis C virus NS5B RNA polymerase displays full functionality
July 30th, 2004
Cloned hepatitis C virus NS5B RNA polymerase displays full functionality. "The NS5B encoded by the hepatitis C virus genome is a RNA-dependent RNA polymerase essential to viral replication. The entire NS5B protein contains a catalytic domain followed by a regulatory motif and a membrane-anchor domain at its C-terminus. Reported here is the molecular cloning and expression of the full-length NS5B polymerase (NS5B-FL) in bacterial cells as a non-fusion protein," scientists in the United States report. "The non-tagged NS5B-FL was purified to homogeneity using sequential chromatographic columns and its identity was confirmed using anti-NS5B peptide...
Source: Genomics & Genetics Weekly (2004-07-30)
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