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Genomics & Genetics Weekly

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Amoebiasis



Polymerase chain reaction repudiates microscopic analyses of enteric amoebas



July 30th, 2004

Polymerase chain reaction repudiates microscopic analyses of enteric amoebas.

According to a study from Ethiopia, "high prevalences of intestinal amoebiasis are commonly reported by microscopy in Ethiopia. In order to confirm the actual occurrence of Entamoeba histolytica we collected 108 stool specimens from different hospitals & health centers from patients in whom haematophagous trophozoites were believed to be found."

"We detected only a single E. histolytica case while 77 (71.3%) were E. dispar and the remaining 30 samples were negative for both species by real-time polymerase chain reaction (PCR) based on the small subunit...


Source: Genomics & Genetics Weekly (2004-07-30)

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