Genomics & Genetics Weekly
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Biotechnology
Biosensors identify DNA binding at the single molecular level
August 6th, 2004
Biosensors identify DNA binding at the single molecular level. According to published research from Japan, "a novel method for detecting interaction between DNA and DNA-binding protein at the single molecular level has been proposed. In this study, estrogen receptor-alpha (ER-alpha) was used for biosensing as the proof-example." "A 518 bp-long (ca.176 nm) DNA probe labeled with streptavidin at its 5'-terminus was prepared by inserting a consensus oligonucleotide sequence that binds to ER-alpha. A solution containing ER-alpha was dropped onto the Ni-treated mica substrate on which the DNA prove was previously immobilized, and it was observed by AFM,"...
Source: Genomics & Genetics Weekly (2004-08-06)
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