Oncology
Microarray discerns DNA changes in microdissected ovarian cancer specimens
Published in Genomics and Genetics Weekly, March 4th, 2005
According to researchers in the United States, "we have established a method for using a cDNA array platform in combination with degenerate oligonucleotide primer polymerase chain reaction (DOP-PCR) and taramide signal amplification (TSA) to identify DNA copy number abnormalities (CNA) in cancer cell lines and cancer cells procured with laser-based microdissection."
"To determine the sensitivity and specificity for detecting single-copy gain and loss, receiver-operator curve analysis was performed on hybridization signal ratios generated from non-DOP and DOP amplified female...
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