Biochemistry
Research on biochemistry detailed by scientists at University of California
Published in Genomics and Genetics Weekly, November 10th, 2006
New research, "Multiple levels of affinity-dependent DNA discrimination in Cre-LoxP recombination," is the subject of a report. "Cre recombinase residue Arg259 mediates a canonical bidentate hydrogen-bonded contact with Gua27 of its LoxP DNA substrate. Substituting Cyt8-Gua27 with the three other basepairs, to give LoxAT, LoxTA, and LoxGC, reduced Cre-mediated recombination in vitro, with the preference order of Gua27 >Ade27 approximately Thy27 >>Cyt27," scientists writing in the journal Biochemistry report.
"While LoxAT and LoxTA exhibited 2.5-fold reduced affinity and 2.5-5-fold slower reaction rates, LoxGC was a barely functional substrate. Its...
Want to see the full article?
We're a pay-per-view site for premium content. If you'd like to purchase this article, it's only $3.00.
Welcome to NewsRx!
Learn more about a six-week, no-risk free trial of Genomics and Genetics Weekly
More Articles from Genomics and Genetics Weekly
- Genetics & Genomics
Heart failure after myocardial infarction improves in absence of NF-kappaB subunit p50 - Prostate Cancer
Prostate cancer laminin genes support signal transduction - Thyroid Cancer
Human microRNA is deregulated in follicular thyroid carcinoma - Thyroid Cancer
Papillary thyroid cancer with BRAF V600E mutation correlates with poorer outcomes - Allergies Gene Therapy
Australian National University, Division of Molecular Biosciences reports research in allergies gene therapy - Cancer Gene Therapy
Researchers at London Health Sciences Center, Department of Surgery release new data on cancer gene therapy - More from this issue of Genomics and Genetics Weekly
NewsRx is Social
Follow us on your favorite social network by clicking on a button below:
Top Medical Newsletters
Awards
Best Health/Healthcare Content, 2012
Best Health/Healthcare Content, 2011
Best e-Business Site, 2010
Best e-Business Site, 2009
Best e-Business Site, 2008
Best e-Business Site, 2007
Best e-Business Site, 2006
Best Healthcare Content, 2005
Best Overall Internet Site, 2005
Best Interactive Site, 2005
Facts & Stats
NewsRx also is available at LexisNexis, Gale, ProQuest, Factiva, Dialog, Thomson Reuters, NewsEdge, and Dow Jones.
- Google 2010 PageRank: #2 Among Top Health News and Media Publications
- Google 2010 PageRank: #2 Among Top Science Publications in Biology/Physiology
- Google 2010 PageRank: #2 Among Top News and Media for the Business of Pharmaceuticals
- Amazon's Alexa 2010 PageRank: #2 News and Media Site for the Pharmaceutical Industry
